Biodiesel production from abundant bio-sources has drawn the attention of the academic as well as the industrial community in recent years.

However, one of the most serious obstacles for using biodiesel as an alternative fuel is the complicated and costly purification processes involved in its production. The difficulties involved in the separation of glycerine and other un-reacted reactants and by-products necessitate the development of new competent low cost separation processes for this purpose. In this work, a low cost quaternary ammonium salt-glycerine-based ionic liquid is proposed as a solvent for extracting glycerine from the transesterification biodiesel product. The separation technique was tested on palm oil-based produced biodiesel with KOH as a reaction catalyst. The lab scale purification experiments proved the viability of the separation technique. The purified biodiesel fulfilled the EN 14214 and ASTM D6751 standard specifications for biodiesel fuel in terms of glycerine content. A continuous separation process is suggested for possible industrial scale application.