Abundance of cellulose is considered to be a promising raw material to produce various products including sugars, single cell proteins, hydrogen, and methane. One of effective ways to break the cellulose chemical structure to sugars is to utilize microbial enzymes. In this research, 57 isolates from Thai higher termites, Microcerotermes sp., classified as cellulase-producing bacteria, were initially identified by measuring hydrolysis capacity value, or HC value. The three highest HC value isolates of Bacillus subtilis (strain A 002, M 015, and F 018) were tested for the specific cellulase enzyme activities—endoglucanase, exoglucanase, and β-glucosidase activity—at 37°C and pH 7.2 for 24 hours. Their tolerance to the presence of the ionic liquid, 1-butyl-3-methylimidazolium chloride, or [BMIM]Cl used to enhance the accessibility of cellulase enzyme by changing cellulose structure in the pretreatment step, was also investigated. The results showed that strain M 015 had the highest specific endoglucanase activity; and strain F 018 had the highest specific exoglucanase activity and β-glucosidase activity. From the tolerance study results, strains M 015 and F 018 were able to tolerate [BMIM]Cl in the range of 0.1 to 1.0 % (v/v) without retarding the lag phase of the growth curve. In contrast, for strain A 002, the growth retardation in the lag phase in the presence of 0.5 to 1.0 %(v/v) of [BMIM]Cl was observed.